| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibodies | -H1 (1:1000; Cell Signaling, Beverly, USA), PAK4 (1:2000; Cell Signaling), RCC2 (1:1000; Abcam), Flag-tag M2 (1:5000; Sigma-Aldrich), GFP-tag (1:5000; GenScript, Nanjing, China), and GAPDH (1:2000; KangChen, Shanghai, China) antibodies overnight at 4 C. Then the PVDF membranes were incubated with HRP-conjugated | Get A Quote |
Gastric cancer is one of the malignant tumors in the world. PAK4 plays an important role in the occurrence and development of gastric cancer, especially in the process of invasion and metastasis. Here we discover that CORO1C, a member of coronin family that regulates microfilament and lamellipodia formation, recruits cytoplasmic PAK4 to the leading edge of gastric cancer cells by C-terminal extension (CE) domain of CORO1C (353 457 aa). The localization of PAK4 on the leading edge of the cell depends on two necessary conditions: the phosphorylation of PAK4 on serine 99 and the binding to the CE domain of CORO1C. Unphosphorylated PAK4 on serine 99 is closely associated with microtubules by PAK4/GEF-H1/Tctex-1 com... More
