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A synthetic biology and green bioprocess approach to recreate agarwood sesquiterpenoid mixtures

green chemistry. 2024-01; 
Sergio Gutiérrez, Sebastian Overmans, Gordon B. Wellman, Vasilios G. Samaras, Claudia Oviedo, Martin Gede, Gyorgy Szekely, Kyle J. Lauersen
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Gene Synthesis This programme back-translates amino-acid sequences to frequently used codons, removes unwanted restriction sites, and systematically integrates the first intron of CrRBCS2i1 at a set distance to enable expression from the C. reinhardtii nuclear genome, as previously reported.49,50 Algal-adapted coding sequences were synthesised and sub-cloned into pOptimized_3 expression plasmids35 by Genscript (Piscataway,NJ, USA). Get A Quote

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