A synthetic biology and green bioprocess approach to recreate agarwood sesquiterpenoid mixtures
| Products/Services Used |
Details |
Operation |
| Gene Synthesis> |
This programme back-translates amino-acid sequences to frequently used codons, removes unwanted restriction sites, and systematically integrates the first intron of CrRBCS2i1 at a set distance to enable expression from the C. reinhardtii nuclear genome, as previously reported.49,50 Algal-adapted coding sequences were synthesised and sub-cloned into pOptimized_3 expression plasmids35 by Genscript (Piscataway,NJ, USA). |
Get A Quote |