| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | The beads were washed five times with Co-IP buffer, denatured in SDS loading buffer, boiled for 10 min, and separated on a 10% SDS–polyacrylamide gel electrophoresis (SDS-PAGE) gel (M00664, GenScript) for immunoblotting. | Get A Quote |
Light undergoes dramatic spectral shifts at dusk (sunset) and dawn (sunrise), enhancing insect activity and increasing the risk of insect-borne virus transmission. However, how plants respond to these light changes and associated viral threats remains unclear. Here, we show that far-red (FR) light, enriched in skylight during dusk and dawn, activates plant antiviral defense via phytochrome A (phyA). FR light-induced phyA activation promotes degradation of phytochrome interacting factor 1 (PIF1), which triggers the REVEILLE 7 (RVE7)-enhanced disease susceptibility 1 (EDS1) cascade to enhance salicylic acid (SA) signaling. Furthermore, multiple viruses use their RNA-dependent RNA polymerases to disrupt the phyA-f... More
