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Catalog Peptides> | To determine whether CPK28 and CRN78 dissociate from PIP2 after nlp20 treatment, protoplasts transfected with 100 mg of the indicated plasmids were treated with 50 mM E64D (MedChemExpress) and 50 mM PS341 (MedChemExpress) for 4 h, followed by treatment with 1 mM nlp20 (Genscript) for the indicated times. | Get A Quote |
Plasma membrane intrinsic proteins (PIPs), a subclass of aquaporins, play an important role in plant immunity by acting as H2O2 transporters. Their homeostasis is mostly maintained by C-terminal serine phosphorylation. However, the kinases that phosphorylate PIPs and manipulate their turnover are largely unknown. Here, we found that Arabidopsis thaliana PIP2;7 positively regulates plant immunity by transporting H2O2. Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28) directly interacts with and phosphorylates PIP2;7 at Ser273/276 to induce its degradation. During pathogen infection, CPK28 dissociates from PIP2;7 and destabilizes, leading to PIP2;7 accumulation. As a countermeasure, oomycete pathogens produ... More