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Effectiveness of six molecular typing methods as epidemiological tools for the study of isolates in two Colombian regions

Vet World. 2019-12; 
Kelly Lozano-Villegas, Roy Rodríguez-Hernández, Iang Rondón-Barragán
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Molecular Biology Reagents … using 18 primers from the GenScript USA Series (Table 1). … The highest genetic similarity is observed 0.813 between (P… , where it is clear that the genetic distance is very high between (G… Get A Quote

摘要

objective: The aim of this study was the genotypic characterization of the strains of spp. isolated from broiler chickens and humans with gastroenteritis from two regions of Colombia, by BOXA1R-polymerase chain reaction (PCR) and random amplification of polymorphic DNA (RAPD)-PCR methods. methods: Forty-nine strains of were assessed, 15 from poultry farms in Santander region, and 34 from Tolima region isolated from poultry farms (n=24) and the stool samples of people with gastroenteritis (n=10). BOXA1R primers were selected for repetitive element-based PCR (REP-PCR) and five arbitrary primers, namely, GTG 5, OPB 15, OPP 16, OPS 11, and P 1254 were used for RAPD-PCR to generate DNA fingerprints from the isolat... More

关键词

dendrogram, serotyping, typing methods