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Oncolytic adenovirus with MUC16-BiTE shows enhanced antitumor immune response by reversing the tumor microenvironment in PDX model of ovarian cancer

Oncoimmunology. 2022-07; 
Qiuman Wang , Xinyue Ma , Huan Wu , Chen Zhao 1, Jingying Chen , Rongrong Li , Shi Yan , Yingwei Li , Qing Zhang , Kun Song , Cunzhong Yuan , Beihua Kong
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Proteins, Expression, Isolation and Analysis The concentration of MUC16-BiTE secreted by HEK293A was determined by His Tag ELISA Detection Kit (Genscript). Get A Quote

摘要

The improved survival rate of ovarian cancer (OC) is related to the action of infiltrating cytotoxic T lymphocytes (CTLs). Recently, oncolytic adenoviruses (OAds) have emerged as a key player in treating solid tumors; however, the immunosuppressive tumor microenvironment (TME) and the body-mediated antiviral immune response limit their therapeutic effect. In this study, we tested the hypothesis that bispecific T-cell engagers (BiTEs) could activate and redirect CTLs to increase the anti-tumor effect of OAds. We modified the parental OAd to express a MUC16-targeting BiTE antibody (OAd-MUC16-BiTE), which retained its oncolytic properties and replication ability in vitro. This BiTE secreted from infected tumor cel... More

关键词

Oncolytic virus; PDX; adenovirus; bispecific T-cell engagers; cytotoxic T lymphocyte; immunotherapy; ovarian cancer; tumor microenvironment.