| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Peptides | … All the peptides were purchased from GenScript. All NMR samples were prepared in 95% H2O/5% D2O (v/v) containing 50 mM potassium phosphate (pH 6.9), 100 mM KCl, 1 mM DTT and 0.1 mM EDTA. To examine CSP and the line broadening of the 1H-15N HSQC spectrum … | Get A Quote |
The endoplasmic reticulum transmembrane protein vesicle-associated membrane protein-associated protein (VAP) plays a central role in the formation and function of membrane contact sites (MCS) through its interactions with proteins. The major sperm protein (MSP) domain of VAP binds to a variety of sequences which are referred to as FFAT-like motifs. In this study, we investigated the interactions of eight peptides containing FFAT-like motifs with the VAP-A MSP domain (VAP-A ) by solution NMR. Six of eight peptides are specifically bound to VAP-A. Furthermore, we found that the RNA-dependent RNA polymerase of severe acute respiratory syndrome coronavirus 2 has an FFAT-like motif which specifically binds to VAP-A ... More
