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Molecular Biology Reagents> | … 2.2. Transfection. With regards to the overexpression of lncRNA GAS5, shRNA was used for up-regulation of lncRNA GAS5. Meanwhile, the mimics and inhibitor of miR-222-3p and miR-NC were designed and compounded by Genscript (Nanjing, China) … | Get A Quote |
Hypoxia may cause diseases in human beings. The up- or down- regulation of lncRNAs were identified to possess the ability to protect the myocardial cells from hypoxia injury. This study explored the role of lncRNA GAS5 in sodium Hydrosulfite Induced Hl-1 Cells in vitro. RT-qPCR was applied to measure the expressions of RNAs whereas the cell viability was detected with CCK-8. Luciferase report assay was performed to validate the binding between lncRNA GAS5 and miR-222-3p. The proteins regarding PI3K/AKT signaling were evaluated through western blot analysis. The results showed that the hypoxia could reduce cell viabilities and enhance the apoptosis. Meanwhile, the PI3K/AKT signaling pathway was suppressed as wel... More