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Invariant chain regulates endosomal fusion and maturation through an interaction with the SNARE Vti1b

J Cell Sci. 2020; 
Azzurra Margiotta, Dominik Frei, Ingrid Hegnes Sendstad, Lennert Janssen, Jacques Neefjes, Oddmund Bakke
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Molecular Biology Reagents … 54759 and # 54945, respectively). His6-Ii p33 wt and His6-Δ27Ii in pCGFP-EU were purchased from GenScript. pCGFP-EU empty vector has been described before (Kawate and Gouaux, 2006). mcherry-Rab5 Q79L, mapple-Rab5, mcherry-Rab5, EGFP- Rab5Q79L and … Get A Quote

摘要

The Invariant chain (Ii, CD74) is a multifunctional regulator of adaptive immune responses and responsible for sorting MHC-I, MHC-II and other Ii-associated molecules to a specific endosomal pathway. When Ii is expressed, endosomal maturation and proteolytic degradation of proteins are delayed and in non-antigen presenting cells the endosomal size increase, but he molecular mechanisms are not known. We identified that a SNARE, Vti1b, is essential for regulating these Ii induced effects. Vti1b binds to Ii and Vti1b is localized at the contact sites of fusing Ii positive endosomes. Furthermore, a tailless Ii that is not internalized from the plasma membrane relocates Vti1b to the plasma membrane. KO of Ii in an a... More

关键词

Endosomal fusion, Ii, MHCII, SNAREs, Vti1b