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A high-throughput assay for the quantification of intact Insulin-like Growth Factor I in human serum using online SPE-LC-HRMS

Clin Chim Acta. 2020; 
Gilles Coppieters, Péter Judák, Nicolas Van Haecke, Pieter Van Renterghem, Peter Van Eenoo, Koen Deventer
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Molecular Biology Reagents … Kingdom). A second standard (NIST Standard Reference Material® 2926) was purchased from the National Institute of Standards & Technology (Gaithersburg, Maryland) and a third from GenScript Biotech (Leiden, Netherlands) … Get A Quote

摘要

Quantification of IGF-I is relevant in both doping control as a biomarker of growth hormone (GH) misuse in sports, and in the clinical field for longitudinal follow-up of patients with disorders related to the GH axis. Currently, better standardization of IGF-I measurements using mass spectrometry is in our best interest as it would enable long-term monitoring of an athletes' IGF-I levels by its addition to the Athlete Biological Passport (ABP). Here, a simplified and rapid top-down LC-HRMS method for quantification of IGF-I in human serum is presented. A ten-minute precipitation-based offline sample preparation is combined with online sample clean-up and separation on a conventional LC, resulting in a total ru... More

关键词

Doping control, Insulin-like Growth Factor I (IGF-I), Liquid Chromatography High-Resolution Mass Spectrometry (LC-HRMS), Quantification, Serum, Top-down