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Evidence of Rubus Yellow Net Virus Integration into the Red Raspberry Genome

Cytogenet Genome Res. 2020; 
Alfredo Diaz-Lara, Nola J Mosier, Kristian Stevens, Karen E Keller, Robert R Martin
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Molecular Biology Reagents … PCR amplification was performed using Taq polymerase (GenScript, Piscataway, NJ, USA; PCR program consisted of 3 min at 95°C; 40 cycles of 40 s at 95°C, 40 s at 56°C, and 40 s at 72°C, with a final elongation step of 10 min at 72°C) and yielded amplicons of about 400 bp … Get A Quote

摘要

Rubus yellow net virus (RYNV) infects Rubus spp., causing a severe decline when present in mixed infections with other viruses. RYNV belongs to the family Caulimoviridae, also known as plant pararetroviruses, which can exist as episomal or integrated elements (endogenous). Most of integrated pararetroviruses are noninfectious; however, a few cases have been reported where they excised from the plant genome and formed infectious particles. Graft transmission onto indicator plants R. occidentalis "Munger" has been the standard test method for RYNV detection in certification programs. Previously, it was noticed that some RYNV PCR-positive plants did not induce symptoms on "Munger", suggesting an integration event.... More

关键词

Rubus spp., Discrimination, Genome, Integrated, Pararetrovirus