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Mechanosensitive regulation of stanniocalcin-1 by zyxin and actin-myosin in human mesenchymal stromal cells

Stem Cells. 2020; 
Jip Zonderland, David B Gomes, Yves Pallada, Ivan L Moldero, Sandra Camarero-Espinosa, Lorenzo Moroni
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Molecular Biology Reagents … The alginate was then modified with (Glycine)4-Arginine-Glycine- Aspartic acid-Serine-Proline (referred to as RGD throughout the text) (Genscript, Piscataway, NJ, USA) to allow cell adhesion by using aque- ous carbodiimide chemistry (EDC chemistry) … Get A Quote

摘要

Stanniocalcin-1 (STC1) secreted by mesenchymal stromal cells (MSCs) has anti-inflammatory functions, reduces apoptosis, and aids in angiogenesis, both in vitro and in vivo. However, little is known about the molecular mechanisms of its regulation. Here, we show that STC1 secretion is increased only under specific cell-stress conditions. We find that this is due to a change in actin stress fibers and actin-myosin tension. Abolishment of stress fibers by blebbistatin and knockdown of the focal adhesion protein zyxin leads to an increase in STC1 secretion. To also study this connection in 3D, where few focal adhesions and actin stress fibers are present, STC1 expression was analyzed in 3D alginate hydrogels and 3D... More

关键词

3D culture, actin-myosin, mechanotransduction, mesenchymal stromal cell, stanniocalcin-1, zyxin