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Antimicrobial polymer-peptide conjugates based on Maximin H5 and PEG to prevent biofouling of E coli and P aeruginosa

ACS Appl Mater Interfaces. 2020; 
Valerie Ortiz Gomez, Victor D Rodriguez-Ramos, Rafael Maldonado-Hernandez, José A Gonzalez-Feliciano, Eduardo Nicolau
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Molecular Biology Reagents … COOH, 2125.54 Da, 95% purity) and Tet-20 (KRWRIRVRVIRKC-COOH, 1769.23 Da, 95% purity) AMPs were purchased from GenScript Co.Dimethyl Sulfoxide (DMSO), molecular biology 99% purity, Tris (2-carboxyethyl) phosphine hydrochloride (TCEP), Dulbecco's … Get A Quote

摘要

Many pathogens, such as Pseudomonas aeruginosa and Escherichia coli bacteria can easily attach to surfaces and form stable biofilms. The formation of such biofilms in surfaces presents a problem in environmental, biomedical, and industrial processes, among many others. Aiming to provide a plausible solution to this issue, the anionic and hydrophobic peptide Maximin H5 C-terminally deaminated isoform (MH5C), has been modified with a cysteine in the C-terminal (MH5C-Cys) and coupled to polyethylene glycol (PEG) polymers of varying sizes (i.e. 2 kDa and 5 kDa) to serve as a surface protective coating. Briefly, the MH5C-Cys was bio-conjugated to PEG and purified by Size Exclusion Chromatography (SEC) while the reac... More

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