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Involvement of nNOS in the antinociceptive activity of melatonin in inflammatory pain at the level of sensory neurons

Eur Rev Med Pharmacol Sci. 2020; 
S-S Xie, W-G Fan, Q Liu, J-Z Li, M-M Zheng, H-W He, F Huang
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Molecular Biology Reagents … Assay Kit (Beyotime, Shanghai, China). De- natured protein was used for 4-20% Bis-Tris sodium dodecyl sulfate polyacrylamide gel electrophoresis (Sure-PAGE, GenScript, Piscataway, NJ, USA). Then, the proteins were electrotransferred … Get A Quote

摘要

objective: The efficacy of melatonin as an analgesic agent has been well documented in animals and humans. However, the underlying mechanisms by which melatonin exerts antinociceptive effects on inflammatory pain are poorly understood. Here, we investigated the potential of melatonin to ameliorate inflammatory pain. methods: In vitro, ND7/23 neurons were treated with capsaicin. We used PCR and Western blot analyses to detect the expression of neuronal nitric oxide synthase (nNOS) in response to melatonin. Orofacial inflammatory pain was induced by 4% formalin administration on the right whisker pad of Sprague Dawley (SD) rats. The analgesic effect of melatonin was evaluated using mechanical threshold analyses. ... More

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