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Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

PLoS ONE. 2020; 
Laidi Wang, Wenshuang Liang, Shasha Wang, Zhixiu Wang, Hao Bai, Yong Jiang, Yulin Bi, Guohong Chen, Guobin Chang
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Molecular Biology Reagents … NC. Western blot analysis. Total protein was extracted from cells using a RIPA buffer (Beyotime) supplemented with a protease inhibitor Cocktail (BioDee) (100:1). Protein was separated on 8% SDS-PAGE gels (GenScript). The … Get A Quote

摘要

Adipocytes are derived from pluripotent mesenchymal stem cells through adipogenesis. Pre-adipocyte differentiation in poultry greatly influences fat deposition and meat quality. Circular RNAs (circRNAs) have an important function in cancer and some differentiation processes. Herein, high-throughput transcriptome sequencing was used to detect circRNAs present in cherry valley duck pre-adipocyte and adipocyte differentiation over 3 days. We identified 9,311 circRNAs and 141 differentially expressed circRNAs. Sequencing results were verified through qRT-PCR using seven randomly selected circRNAs, and competing endogenous RNA (ceRNA) networks were exhibited by ten important circRNAs in duck adipocyte differentiatio... More

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