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Molecular Biology Reagents> | … Immunoblot Analysis Cells were lysed in High Salt ELB as described previously (Attucks et al., 2014) and electrophoresed through a Genscript ExpressPlusTM PAGE Precast gel and transferred onto 0.45 μm nitrocellulose membrane (BioRad, Hercules, CA) … | Get A Quote |
Biosynthesis and degradation of heme, an iron-bound protoporphyrin molecule utilized by a wide variety of metabolic processes, are tightly regulated. Two closely related enzymes, heme oxygenase 1 (HMOX1) and heme oxygenase 2 (HMOX2), degrade free heme to produce carbon monoxide, Fe , and biliverdin. HMOX1 expression is controlled via the transcriptional activator, NFE2L2, and the transcriptional repressor, Bach1. Transcription of HMOX1 and other NFE2L2-dependent genes is increased in response to electrophilic and reactive oxygen species. Many tumor-derived cell lines have elevated levels of NFE2L2. Elevated expression of NFE2L2-dependent genes contributes to tumor growth and acquired resistance to therapies. He... More