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Molecular Biology Reagents> | … Equivalent amounts of protein were separated on a 4–12% SurePAGE gel (GenScript, Nanjing, China). After electrophoresis, the separated proteins were placed onto a nitrocellulose membrane (Pall, Mexico), and then inhibited with 5% (w/v) skimmed milk … | Get A Quote |
miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of bPCs. Luciferase reporter assays showed that the 3'-UTR region of (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of by siRNA facilitated the MD of bPCs, while ... More