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Imperatorin Relieved Ulcerative Colitis by Regulating the Nrf-2/ARE/HO-1 Pathway in Rats

Inflammation. 2020; 
Min Luo, Yin Luo
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Molecular Biology Reagents … The probes of ARE were obtained from the Genscript company (Nanjing, China). Twenty-microgram samples of the nuclear proteins were incubated with the probes for 20 min at 25 °C, followed by electrophoresis using 6% polyacrylamide gel … Get A Quote

摘要

Ulcerative colitis is a common intestinal inflammatory disease. Imperatorin (IMP) has been reported to alleviate mast cell-mediated allergic responses by suppressing the expression of Nrf-2, ARE, and HO-1. However, whether IMP can relieve ulcerative colitis by regulating Nrf-2/ARE/HO-1 pathway is unclear. Thus, this study aims to investigate the effect of IMP on ulcerative colitis in rats and elucidate the potential mechanism. In our study, rats were treated with 2,4,6-trinitro-benzenesulfonic acid (TNBS) to induce the animal model of ulcerative colitis. Next, these rats were treated with diverse doses of IMP (15 mg/kg, 30 mg/kg, and 60 mg/kg) and sacrificed at different time points (3 days, 7 days, and 14... More

关键词

Nrf-2/ARE/HO-1 pathway, imperatorin, inflammation, ulcerative colitis