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Programmed death ligand-1 induction restrains the cytotoxic T lymphocyte response against microglia

Glia. 2020; 
Priyanka Chauhan, Shuxian Hu, Sujata Prasad, Wen S Sheng, James R Lokensgard
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Molecular Biology Reagents … Neurovirology Laboratory, Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA … from MBL Life Science (Sunnyvale, CA), while YSPTSILDI (ie, YI9; Gag 277–285 ) and SIINFEKL (ie, SL8; OVA 257–264 ) were bought from GenScript (Piscataway … Get A Quote

摘要

Microglial cells are the main reservoir for HIV-1 within the brain and potential exists for negative immune checkpoint blockade therapies to purge this viral reservoir. Here, we investigated cytolytic responses of CD8 T lymphocytes against microglia loaded with peptide epitopes. Initially, flow cytometric analysis demonstrated efficient killing of HIV-1 p24 AI9 or YI9 peptide-loaded splenocytes in MHC-matched recipients. Cytolytic killing of microglia was first demonstrated using ovalbumin (OVA) as a model antigen for in vitro cytotoxic T lymphocyte (CTL) assays. Peptide-loaded primary microglia obtained from programmed death ligand (PD-L) 1 knockout (KO) animals showed significantly more killing than cells fro... More

关键词

HIV-associated neurocognitive disorders, brain-resident memory T-cells, cytotoxic T lymphocyte, microglia, programmed death (PD)-1: PD-L1 pathway