| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … transposable vectors pSBbi-Hyg and pSBbi-Pur, respectively (gifts of Eric Kowarz, Addgene plasmids #60524 and #60523) by GenScript (Piscataway, NJ, USA). pSBbi-Hyg-APP was cotransfected with the transposase- encoding … | Get A Quote |
The organic anion transporter Adenosine triphosphate Binding Cassette subfamily C member 1 (), also known as , has been demonstrated in murine models of Alzheimer's disease (AD) to export amyloid beta (Abeta) from the endothelial cells of the blood-brain barrier to the periphery, and that pharmaceutical activation of ABCC1 can reduce amyloid plaque deposition in the brain. Here, we show that ABCC1 is not only capable of exporting Abeta from the cytoplasm of human cells, but also that it's overexpression significantly reduces Abeta production and increases the ratio of alpha- versus beta-secretase mediated cleavage of the Amyloid Precursor Protein (APP), likely via indirect modulation of alpha-, beta-, and gamma... More
