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MiR-34a-5p inhibition attenuates LPS-induced endothelial cell injury by targeting FOXM1

Eur Rev Med Pharmacol Sci. 2020; 
F-M Zhang, W-H Zheng, H-J Wang
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Molecular Biology Reagents … Expression Vector Construction and Cell Transfection MiR-34a-5p mimic, inhibitor, and scramble miRNAs (NC-miRNAs) were obtained from GenScript (Nanjing, China) and used to study the expression of miR-34a-5p in HUVECs … Get A Quote

摘要

objective: This study aims to investigate the role of miR-34a-5p in the regulation of lipopolysaccharide (LPS)-induced injury of vascular endothelial cells (ECs). methods: Human umbilical vein ECs (HUVECs) were exposed to LPS to stimulate endothelial injury in vitro. miRNA microarray analysis was carried out to identify miR-34a-5p expression in HUVECs. MTT and flow cytometry analyses were used to determine cell viability and apoptosis rate, respectively. Furthermore, enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and Western blot were used to examine the factors involved in inflammation and their relative gene expression. Additionally, Matrigel-based tube formation assay was carried out to assess the vascu... More

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