| Products/Services Used | Details | Operation |
|---|---|---|
| Monoclonal Antibody Services | … GFP monoclonal antibody (Genscript, China) was used to detect the protein of GFP-Atg8 and its degradation product GFP. The tubulin monoclonal antibody (Novus Biologicals, USA) was used to examine the α-tubulin as a control. The detection of ATP … | Get A Quote |
Inositol polyphosphate kinases are involved in regulation of many cellular processes in eukaryotic cells. In this study, we investigated the functions of the inositol polyphosphate kinase Vip1 in autophagy and pathogenicity of . Loss of Vip1 caused significantly increased sensitivity to nitrogen source starvation, abnormal localization and degradation of autophagy protein, higher vacuolar pH and higher (rather than lower) intracellular ATP levels compared with control strains. Besides, the mutant showed attenuated hyphal development and virulence during systemic infection to mice. The results reveal that Vip1 is important to autophagy of . The maintenance of vacuolar acidic pH contributed to the role of Vip1 ... More
