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Neuroprotective effects of Fomes officinalis Ames polysaccharides on Aβ-induced cytotoxicity in PC12 cells through suppression of mitochondria-mediated apoptotic pathway

Cytotechnology. 2020; 
Ayijiang Habaike, Mirensha Yakufu, Yuanyuan Cong, Yimin Gahafu, Zhen Li, Palida Abulizi
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Molecular Biology Reagents … Agents Ab25–35 (Genscript, China) was diluted to a final concentration of 2 mM using double-distilled deion- ized water … cells, purchased from Cell Bank of the Chinese Academy of Science (Shanghai, China), were cultured in DMEM (Hyclone, USA) supplemented with 10 … Get A Quote

摘要

Aggregation of Aβ is a pathological hallmark of Alzheimer's disease (AD). The purpose of this study was to identify the protective roles of different polysaccharide components in Fomes officinalis Ames polysaccharides (FOAPs) against Aβ-induced neurotoxicity in PC12 cells. Different doses of FOAPs components (i.e. FOAPs-a and FOAPs-b) were added to PC12 cells about 2 h before β-amyloid protein fragment 25-35 (Aβ) exposure. The AD cellular model of PC12 cells was established using Aβ. Then the PC12 cells were divided into 9 groups including: control group, Donepezil hydrochloride (DHCL) group, model group treated using 40 μM Aβ, followed by FOAPs-a and FOAPs-b interference (50, 100 and 200 μg/mL). The... More

关键词

Aβ25–35, Fomes officinalis Ames polysaccharides, Mitochondrial dysfunction, Oxidative stress, PC12 cells