Products/Services Used | Details | Operation |
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Custom Vector Construction> | … incorporated as shown in Fig. 1. Following commercial synthesis (http://www.genscript. com/), these two genes were inserted into the commercial vector pIRES (Clontech Laboratories, Inc, USA). TOP10 chemically competent E … | Get A Quote |
background: Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. methods: In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine wa... More