| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … The plasmid with rMIC1(T126A/T220A), carrying mutations into the CRDs of MIC1 that compromise its lectin property [17], can be synthe- sized by GenScript (NJ, USA) using a pET28a vector. 398 Fla´ via Costa Mendonc¸ a-Natividade et al. Page 9 … | Get A Quote |
Some lectins of pathogens interact with host cells through the recognition of specific carbohydrates displayed on the mammals' cell surface. The microneme protein 1 (MIC1) from Toxoplasma gondii has a lectin domain that specifically binds sialic acid residues, often found in the terminal positions of N-glycans of mammalian cells. The necessary studies on the MIC1 biological roles have been limited initially by the laborious purification of the protein from T. gondii tachyzoites and the low yields verified. Then Escherichia coli has been transformed with a construct containing the MIC1 gene, and the obtained recombinant MIC1 (rMIC1) has been purified from the inclusion bodies. Herein, we detail the methodology o... More
