| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … down seperately. The WS/DGAT gene from A. baylyi ADP1 was synthesized and provided by GenScript (Nanjing, China) (Table S2). The WS/DGAT was amplified with the primers WS/DGAT-up and WS/DGAT-down. The pET28a … | Get A Quote |
Fatty acid short-chain esters (FASEs) are biodiesels that are renewable, nontoxic, and biodegradable biofuels. A novel approach for the biosynthesis of FASEs has been developed using metabolically-engineered E. coli through combination of the fatty acid and 2-keto acid pathways. Several genetic engineering strategies were also developed to increase fatty acyl-CoA availability to improve FASEs production. Fed-batch cultivation of the engineered E. coli resulted in a titer of 1008 mg/L FASEs. Since the fatty acid and 2-keto acid pathways are native microbial synthesis pathways, this strategy can be implemented in a variety of microorganisms to produce various FASEs from cheap and readily-available, renewable, raw... More
