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WT1-AS promotes cell apoptosis in hepatocellular carcinoma through down-regulating of WT1

J Exp Clin Cancer Res. 2015-10-01; 
Long Lv, Gong Chen, Jianping Zhou, Jun Li, Jianping Gong
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Custom Vector Construction … The secondary antibodies were anti-rabbit or anti-mouse HRP-linked were purchased from Santa Cruz Biotechnology (CA, USA) … or a mutated sequence with the predicted target sites were inserted into the KpnI and SacI sites of pGL3 promoter vector (Genscript, Nanjing, China … Get A Quote

摘要

background: The antisense of the tumor suppressor gene WT1 (WT1-AS) is a long non-coding RNA. The role of WT1-AS in the development of hepatocellular carcinoma (HCC) has not yet been elucidated. methods: Quantitative real-time PCR and western blot analyses were used to measure levels of WT1-AS and its related genes in tumor and corresponding adjacent tumor tissues of HCC patients. The effect on HCC cell proliferation and apoptosis was assessed by EdU incorporation assays and PI-Annexin-V staining, respectively. ShRNA and dual-luciferase assays were used to investigate the regulatory relationship between WT1-AS and WT1 in cell lines. results: WT1-AS expression correlated negatively with WT1 expression in HCC tum... More

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