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Histological characterization of orphan transporter MCT14 (SLC16A14) shows abundant expression in mouse CNS and kidney

BMC Neurosci. 2016-07-01; 
Sahar Roshanbin, Frida A Lindberg, Emilia Lekholm, Mikaela M Eriksson, Emelie Perland, Johan Åhlund, Amanda Raine, Robert Fredriksson
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Custom Vector Construction … Cells were transfected with one µg of a pcDNA3.1 + DYK vector containing the full Slc16a14 mRNA sequence, as well as a FLAG tag sequence (Additional file 3: Figure S2, DYKDDDK, Clone ID: OMu04791, Genscript Piscataway, NJ, USA), using Lipofectamine Plus reagent … Get A Quote

摘要

background: MCT14 (SLC16A14) is an orphan member of the monocarboxylate transporter (MCT) family, also known as the SLC16 family of secondary active transmembrane transporters. Available expression data for this transporter is limited, and in this paper we aim to characterize MCT14 with respect to tissue distribution and cellular localization in mouse brain. results: Using qPCR, we found that Slc16a14 mRNA was highly abundant in mouse kidney and moderately in central nervous system, testis, uterus and liver. Using immunohistochemistry and in situ hybridization, we determined that MCT14 was highly expressed in excitatory and inhibitory neurons as well as epithelial cells in the mouse brain. The expression was ex... More

关键词

Amino acid, Monocarboxylate, Mouse, Transporter