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LINC00261 inhibits progression of pancreatic cancer by down-regulating miR-23a-3p

Arch Biochem Biophys. 2020-06-01; 
Xin Wang, Xiaozhuo Gao, Jiaxun Tian, Rui Zhang, Yun Qiao, Xiangdong Hua, Gang Shi
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Molecular Biology Reagents … A total of 1 μg RNA was reversely transcribed to cDNA with specific reverse transcription kit (FulenGen, Guangzhou, China). The cDNA was diluted and qRT-PCR was performed using SYBR mix (Takara) as well as specific primers (Genscript, Nanjing, China) … Get A Quote

摘要

Long noncoding RNAs (lncRNAs) are usually dysregulated in the progression of pancreatic cancer. This research aims to explore the function and mechanism of LINC00261 in pancreatic cancer cell viability, invasion and apoptosis. Cancer Genome Atlas (TCGA) database was applied to analyze the association between survival probability of patients and level of LINC00261 or miR-23a-3p in pancreatic cancer. Quantitative reverse transcription polymerase chain reaction was conducted to analyze the levels of LINC00261 and miR-23a-3p. Cell viability, invasion and apoptosis of pancreatic cancer cells were determined via MTT, transwell invasion assay, and flow cytometry, respectively. The target relationship between LINC00261... More

关键词

Apoptosis, Invasion, LINC00261, Pancreatic cancer, Viability, miR-23a-3p