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Production of rhamnolipids-producing enzymes of Pseudomonas in E coli and structural characterization

Higher Education Press and Springer-Verlag Berlin Heidelberg. 2017; 
Kata Kiss, Wei Ting Ng, Qingxin Li
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Gene Synthesis … 2 Materials and methods 21 Protein expression The cDNAs encoding RhlA, RhlB, RhlC, RhlR and RhlI were synthesized by Genscript and cloned into NdeI and XhoI sites of pET29b, respectively All the gene sequences were confirmed by DNA sequencing … Get A Quote

摘要

Rhamnolipids are a class of biosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in Pseudomonas aeruginosa. Four of the 5 proteins except RhlC were successfully over-expressed in E. coli and three of them (RhlA, RhlB and RhlI) were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins in vitro to favor the construction of super strains for rhamnolipids production.

关键词

rhamnolipids, Pseudomonas, RhlA, RhlB, RhlI, protein folding
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