| Products/Services Used | Details | Operation |
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| Gene Synthesis | Peptide stabilization of HLA-C was assessed by flow cytometry largely as described (Sim et al., 2017). 105 cells were incubated overnight at 26°C with 100 μM of synthetic peptide. The following day cells stained with APC HLA-I mAb (W6/32, Biolegend) at 4°C. Peptides were synthesized by Genscript (USA). | Get A Quote |
The killer-cell Ig-like receptor (KIR) family, expressed mainly in natural killer (NK) cells, includes an activation receptor of unknown function, KIR2DS4. Here we show that KIR2DS4 is restricted by HLA-C*05:01 with a strong preference for tryptophan at position 8 of 9-mer peptides. ‘Self’ peptides with Trp8 eluted from HLA-C*05:01 are rare and only one out of 12 bound KIR2DS4. An HLA-C*05:01-peptide complex that bound KIR2DS4 was sufficient for strong activation of primary KIR2DS4+ NK cells, independently of coactivation by other receptors and of prior NK cell licensing. A highly conserved sequence in bacterial recombinase A, which is essential for DNA repair and survival, includes an epitope that bound t... More
