| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Two transcripts of rat FGF5 have been reported previously, the short one lacking exon2, and two transcripts have the same nucleotide sequences at both ends (Hattori et al., 1996). Hence, one pair of primers (Table 1) was designed to amplify the complete coding sequence (CDS) of the cgFGF5 gene according to the cattle FGF5sequences retrieved from NCBI GenBank (accession number: EF042192.1). PCR reactions were performed in 25 μl reaction volume containing 2.5 μl 10× Taq Buffer, 2 μl 25 mM MgCl2, 2.5 μl 2 mM dNTP, 0.8 μl each primer (10 μM), 1.0 U Taq DNA polymerase and 60 ng cDNA. The PCR products were then separated by 1.5% agarose gel electrophoresis, and purified by a Gel Extraction Kit (Tiangen, Beijing, China). The purified PCR products were cloned into the pMD19-T vector (TaKaRa Biotechnology, China) and sequenced by GenScript Co., Ltd. (Nanjing, China). | Get A Quote |
Fibroblast growth factor 5 (FGF5) is a secreted signaling protein which has been proved to inhibits hair growth and induces catagen in mouse hair follicle in vivo. Shaanbei white cashmere goat (SWCG), a unique genetic resource in China, was used as the subject in this study. The cDNA of FGF5 of SWCG (cgFGF5) was firstly cloned. The length of the open reading frame (ORF) in cgFGF5 was 813 bp, encoding a protein of 270 amino acid residues. Our results showed that the cgFGF5 gene expressed one alternative variant, designated as cgFGF5s, which was derived from the cgFGF5 complete transcript via alternative splicing (AS). Analyses of the putative proteins sequences revealed that the same signal peptide was ... More
