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Molecular characterization of a MYB protein from Oryza sativa for its role in abiotic stress tolerance

Brazilian Archives of Biology and Technology. 2017; 
Farah Deeba Tasawar Sultana Bushra Javaid Tariq Mahmood S. M. S. Naqvi
Products/Services Used Details Operation
Custom Vector Construction The NCBI database was used for searching OsMYB1 nucleotide and protein sequences and CLUSTALW for sequence alignment. SMART (http://smart.embl-heidelberg.de/) online tool was used to predict conserve DNA binding domain. Secondary structure was predicted by PSIPRED Protein Structure Prediction server (http://bioinf.cs.ucl.ac.uk/psipred/). Nuclear localization signals (NLS) prediction was carried out using online tool WoLF pSORT (http://www.genscript.com/wolf-psort.html). Primary sequence was analyzed by DISOPRED (http://bioinf.cs.ucl.ac.uk/psipred/?disopred=1) to measure the degree of intrinsic disorder. Protein-protein interaction network was constructed by STRING (http://string-db.org/newstring_cgi/show_input_page.pl?UserId=TwZ3I284aTRf&sessionId=AzWVpTDVECpt). The interactions comprise of physical and functional combinations. Get A Quote

摘要

The NCBI database was used for searching OsMYB1 nucleotide and protein sequences and CLUSTALW for sequence alignment. SMART (http://smart.embl-heidelberg.de/) online tool was used to predict conserve DNA binding domain. Secondary structure was predicted by PSIPRED Protein Structure Prediction server (http://bioinf.cs.ucl.ac.uk/psipred/). Nuclear localization signals (NLS) prediction was carried out using online tool WoLF pSORT (http://www.genscript.com/wolf-psort.html). Primary sequence was analyzed by DISOPRED (http://bioinf.cs.ucl.ac.uk/psipred/?disopred=1) to measure the degree of intrinsic disorder. Protein-protein interaction network was constructed by STRING (http://string-db.org/newstring_cgi/show_input... More

关键词

abiotic stress; E. coli; Oryza sativa; OsMYB1; pGEX; Real time qPCR