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RNA Interference as a Tool to Selectively Down-Modulate Protein Function

Brain Energy Metabolism. 2014-07; 
Seila Fernandez-FernandezJuan P. Bolaños
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Custom Vector Construction … fluorophores). 5.3 GenScript. GenScript Web page is devoted to shRNA expression based-vectors, although they refer their products as siRNA. GenScript siRNA Design Center requires login and use 21-nt long sequences by default [51] … Get A Quote

摘要

RNA interference (RNAi) is a rapid and efficient technique to cause highly selective protein loss of function in cells. In this chapter, we would like to describe all necessary steps to implement this approach for the first time in a laboratory that is not used to deal with gene databases information and related technical skills. Using either rational criteria or sophisticated—otherwise straightforward—online software, it is now reasonably feasible for everyone to design the small interfering RNA (siRNA) or the short hairpin RNA (shRNA) molecules to knockdown—or “silence”—your protein of interest. We provide recommendations on how to retrieve the mRNA sequence of your protein of interest from the on... More

关键词

RNA interference Small interfering RNA Short hairpin RNA Transfection methods Transfection efficiency Neurons