| Products/Services Used | Details | Operation |
|---|---|---|
| Custom DNA/RNA Oligos | Oligonucleotides (the sequences were listed in Table 1) were obtained from Genscript Biotechnology Co. Ltd. (Nanjing, China). Exonuclease III (Exo III) was obtained from New England Biolabs (Beverly, MA). NF-κB p50 was obtained from Promega (Madison, WI) for used as the standard. Human NF-κB p50 Transcription Factor Assay kit (ab133105) were purchased from Abcam (Cambridge, MA). HeLa cells for used for the real sample assay were purchased from KeyGen Biotech. Co. Ltd. (Nanjing, China). Solutions in our strategy were prepared using Millipore ultrapure water (>18 MΩ cm, Milli-Q). | Get A Quote |
Herein, we report a new probe for the determination of the concentration of NF-κB p50, one kind of DNA-binding transcription factors (TFs), by using Exonuclease III (Exo III)-aided amplification and gold nanoparticle mediated fluorescence intensity. Since TFs play critical roles in various biological processes, the detection of TFs can provide a lot of useful biological information for studding gene expression regulation related disease. In our system, in the presence of transcription factor, Exo III based amplification reaction was trigged. This enzymatic digestion results in the release of intermediate DNA and ultimately liberating the fluorophore (which, separated from the quencher of AuNP and BHQ2, now flu... More
