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Salt‐induced phase separation of CHAPS and potential application for hydrophobic molecule extraction

Journal of Chemical Technology & Biotechnology. 2019-03;

Ai A Lian Soo C Tan Toshihiro Nagamine Mervyn WO Liew

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Gene Synthesis	… between the MRP and MPP Expression and preparation of CYP 2C19 solubilizates The cDNA of CYP 2C19 was synthesized and cloned into pFastBac 1 donor plasmid between XhoI and HindIII restriction sites (Genscript, USA) Recombinant baculovirus was then …	Get A Quote

摘要

3‐[(3‐Cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS) is a zwitterionic detergent commonly utilized in protein biochemistry‐based applications. This study demonstrates for the first time the successful phase separation of CHAPS via salt addition. Elucidation of consolute solution temperature behavior and determination of binodal curve and tie‐line length (TLL) enabled the subsequent development of CHAPS‐based phase separation systems. The systems were then evaluated using a low‐molecular‐weight dye, Coomassie Brilliant Blue (CBB), and integral membrane protein, cytochrome P450 (CYP) 2C19.