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A novel pH-stable, endoglucanase (JqCel5A) isolated from a salt-lake microorganism, Jonesia quinghaiensis

Electronic Journal of Biotechnology. 2016-11; 
LingLin,XiaozhouLiu,YatingZhou,LinyanGuan,JiajiaHe,WeiqianHuang
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Custom Vector Construction … COVID-19 campus closures … obtained PCR products were purified with the AxyPrep DNA purification kit (Axygen) and cloned into the expression vector, pET-28a, which was further transformed into the competent cells of E. coli DH5α and submitted to Genscript (Nanjing, China … Get A Quote

摘要

Endoglucanase, one of three type cellulases, can randomly cleave internal ??-1,4-linkages in cellulose polymers. Thus, it could be applied in agricultural and industrial processes.

关键词

Protein modelingSite-directed mutagenesisCellulasesRecombinant endoglucanase geneCatalytic domain of glycoside hydrolaseCarbohydrate-binding moduleHigh pH stabilityTolerance to deleterious chemicalsTolerance to heavy metalsTolerance to detergents