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Molecular determinants and heterogeneity of tissue-resident memory CD8+ T lymphocytes revealed by single-cell RNA sequencing

biorxiv. 2020; 
Nadia S. Kurd,  Zhaoren He,  J. Justin Milner,  Kyla D. Omilusik,  Tiani L. Louis,  Matthew S. Tsai,  Christella E. Widjaja,  Jad N. Kanbar,  Jocelyn G. Olvera,  Tiffani Tysl,  Lauren K. Quezada,  Brigid S. Boland,  Wendy J. Huang,  Cornelis Murre,  Ananda W. Goldrath,  Gene W. Yeo,  John T. Chang
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摘要

During an immune response to microbial infection, CD8+ T cells give rise to distinct classes of cellular progeny that coordinately mediate clearance of the pathogen and provide long-lasting protection against reinfection, including a subset of non-circulating tissue-resident memory (TRM) cells that mediate potent protection within non-lymphoid tissues. Here, we utilized single-cell RNA-sequencing to examine the gene expression patterns of individual CD8+ T cells in the spleen and small intestine intraepithelial lymphocyte (siIEL) compartment throughout the course of their differentiation in response to viral infection. These analyses revealed previously unknown transcriptional heterogeneity within the siIEL C... More

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