Products/Services Used | Details | Operation |
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Catalog Peptides> | For assessment of cytokine production, cells were cultured in the presence of LCMV GP33-41 peptide (GenScript) and Protein Transport Inhibitor Cocktail (ThermoFisher Scientific) for 3 hours at 37°C. | Get A Quote |
During an immune response to microbial infection, CD8+ T cells give rise to distinct classes of cellular progeny that coordinately mediate clearance of the pathogen and provide long-lasting protection against reinfection, including a subset of non-circulating tissue-resident memory (TRM) cells that mediate potent protection within non-lymphoid tissues. Here, we utilized single-cell RNA-sequencing to examine the gene expression patterns of individual CD8+ T cells in the spleen and small intestine intraepithelial lymphocyte (siIEL) compartment throughout the course of their differentiation in response to viral infection. These analyses revealed previously unknown transcriptional heterogeneity within the siIEL C... More