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Molecular Biology Reagents> | The codon optimized, intron containing gene was chemically synthesized (GenScript, Piscataway Township, New Jersey) and cloned between BamHI-BglII or Eco32I-EcoRI in the pOpt2_mVenus_Paro vector (Wichmann et al., 2018). | Get A Quote |
Survival under excess light conditions requires the light-induced accumulation of protein LHCSR3 and other photoprotection factors, to enable efficient energy-dependent quenching in the green microalga Chlamydomonas reinhardtii. Here, we demonstrate that the high light-tolerant phenotype of mutant hit1 is caused by a de-repression of promoters belonging to photoprotection genes, which in turn results from an inactivation of the E3 ubiquitin ligase substrate adaptor LRS1. Transcriptome analyses of hit1 revealed massive alterations of gene expression modulation as a consequence of perturbed LRS1 function, indicating its role as a crown regulator. In conjunction with random forest-based network modeling, thes... More