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Regulation of the MLH1-MLH3 endonuclease in meiosis

biorxiv. 2020; 
Elda Cannavo,  Aurore Sanchez,  Roopesh Anand,  Lepakshi Ranjha,  Jannik Hugener,  Céline Adam,  Ananya Acharya,  Nicolas Weyland,  Xavier Aran-Guiu,  Jean-Baptiste Charbonnier,  Eva R. Hoffmann,  Valérie Borde,  Joao Matos,  Petr Cejka
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Molecular Biology Reagents To prepare the hMSH4-hMSH5 expression vector, the hMSH4-STREP and hMSH5-8xHIS constructs were codon-optimized for expression in Spodoptera frugiperda Sf9 cells and synthesized (GenScript). Get A Quote

摘要

During prophase of the first meiotic division, cells deliberately break their DNA. These DNA breaks are repaired by homologous recombination, which facilitates proper chromosome segregation and enables reciprocal exchange of DNA segments between homologous chromosomes, thus promoting genetic diversity in the progeny1. A successful completion of meiotic recombination requires nucleolytic processing of recombination intermediates. Genetic and cellular data implicated a pathway dependent on the putative MLH1-MLH3 (MutLγ) nuclease in generating crossovers, but mechanisms that lead to its activation were unclear2–4. Here, we have biochemically reconstituted key elements of this pro-crossover pathway. First, we sh... More

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