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Complement factor B knockdown by short hairpin RNA inhibits laser-induced choroidal neovascularization in rats

Int J Ophthalmol. 2020; 
Xin Wang, Qing-Li Shang, Jing-Xue Ma, Shu-Xia Liu, Cai-Xia Wang, Cheng Ma
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Molecular Biology Reagents The shRNA sequences synthesized by Sangon Biotech Inc. (Shanghai, China) were cloned into a pRNAT-U6.1/ Neo shRNA expression vector (GenScript, Piscataway, NJ, USA) digested with BamHI and HindIII. Get A Quote

摘要

AIM: To evaluate whether recombinant complement factor B (CFB) short hairpin RNA (shRNA) reduces laserinduced choroidal neovascularization (CNV) in rats. METHODS: Laser-induced rat CNV model was established, and then the animals underwent fundus fluorescence angiography (FFA) and hematoxylin and eosin (HE) staining. On day 3 and 7 after photocoagulation, the expression of CFB and membrane attack complex (MAC) was detected by immunhischemistry. A recombinant CFBshRNA plasmid was constructed. CFB and scrambled shRNA plasmids were intravenous injected into rats via the tail vein on the day of laser treatment, respectively. On day 7, the incidence of CNV was determined by FFA, and the expression of CFB and vascular... More

关键词

choroidal neovascularization; complement factor B; short hairpin RNA; membrane attack complex; vascular endothelial growth factor