| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | An Ngb gene corresponding to the sequence of the cDNA clone, which contains the TAT PTD sequence, was chemically synthesized with 5′ NcoI and 3′ BamHI sites as well as codon optimization for expression in E. coli and was subcloned into the EcoRV site of pUC57-Kan (GenScript, China). | Get A Quote |
Neurological diseases have a close relationship to excessive reactive oxygen species (ROS). Neuroglobin (Ngb), an intrinsic protective factor, protected cells from hypoxic/ischemic injury. In the present, we reported a novel neuroprotective manganese porphyrin reconstituted metal protein, Mn-TAT PTD-Ngb, consisting of a HIV Tat protein transduction domain sequence (TAT PTD) attached to the N-terminal of apo-Ngb. Mn-TAT PTD-Ngb had a stronger ROS scavenging ability than that of TAT PTD-Ngb, and reduced intracellular ROS production and restored the function of the mitochondria and inhibited the mitochondria-dependent apoptosis. Besides, Mn-TAT PTD-Ngb activated the phosphoinositide-3 kinase (PI3K)/Akt signaling p... More
