至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

MiR-196b-5p regulates the proliferation of drug-resistant hepatocellular carcinoma cell lines by activating NFkB/ABCB1 signaling pathway

Tropical Journal of Pharmaceutical Research. 2019; 
Bangming Pu , Yong Cao , Yan Li , Li Tang , Jiyi Xia , Bo Li
Products/Services Used Details Operation
Molecular Biology Reagents HepG2 cells were transfected with a miR-196b- 5p inhibitor, miR-196b-5p mimic, or empty vector (GenScript) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and Opti-MEM reagents (Gibco; Thermo Fisher Scientific). The cells were also transfected with pcDNA-NFκBIA (NFκBIA)/NFκBIA-siRNA (NFκBIA-RNAi) or pcDNA-NFκB1 (NFκB1)/NFκB1-siRNA (NFκB1- RNAi). Get A Quote

摘要

Purpose: To explore the molecular function of miR-196b-5p in hepatocellular carcinoma (HCC). Methods: MiR-196b-5p expression levels in HCC tissue samples were assessed by qRT-PCR. MiR- 196b-5p was knocked-down or over-expressed in HepG2 cells by transfecting the cells with plasmids expressing either a miR-196b-5p inhibitor or mimic, respectively, while cell proliferation was assessed by MTT assay. The interaction of miR-196b-5p with target molecules was confirmed using luciferase reporter assay. Cell cycle was investigated by flow cytometry, while NFκBIA expression was assessed by western blotting. Results: MiR-196b-5p was over-expressed in HCC, and miR-196b-5p expression levels in patients with HCC were relat... More

关键词