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Molecular Biology Reagents> | HepG2 cells were transfected with a miR-196b- 5p inhibitor, miR-196b-5p mimic, or empty vector (GenScript) using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) and Opti-MEM reagents (Gibco; Thermo Fisher Scientific). The cells were also transfected with pcDNA-NFκBIA (NFκBIA)/NFκBIA-siRNA (NFκBIA-RNAi) or pcDNA-NFκB1 (NFκB1)/NFκB1-siRNA (NFκB1- RNAi). | Get A Quote |
Purpose: To explore the molecular function of miR-196b-5p in hepatocellular carcinoma (HCC). Methods: MiR-196b-5p expression levels in HCC tissue samples were assessed by qRT-PCR. MiR- 196b-5p was knocked-down or over-expressed in HepG2 cells by transfecting the cells with plasmids expressing either a miR-196b-5p inhibitor or mimic, respectively, while cell proliferation was assessed by MTT assay. The interaction of miR-196b-5p with target molecules was confirmed using luciferase reporter assay. Cell cycle was investigated by flow cytometry, while NFκBIA expression was assessed by western blotting. Results: MiR-196b-5p was over-expressed in HCC, and miR-196b-5p expression levels in patients with HCC were relat... More