| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The amino acid sequence, protein molecular weight, isoelectric point, stability index, and hydrophobicity of the BocLCYb gene were analyzed and predicted by ExPASy (http://web.expasy.org) and NCBI (https://www.ncbi.nlm.nih.gov/). Subcellular localization was predicted by WoLF PSORT (http://www.genscript.com/wolf-psort.html). The conserved domain were predicted by NCBI (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi). Phylogenetic tree analysis of the LCYb proteins was executed in MEGA 6.0 using the neighbor-joining (NJ) method. | Get A Quote |
Lycopene β-cyclase (LCYb) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata LCYb (BocLCYb) gene sequence was obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocLCYb gene mapped to Scaffold000212, and contains an open reading frame of 1,500 bp that encodes a 499-amino acid protein with a calculated molecular mass of 50.90 kD and an isoelectric point (pI) of 6.77. Subcellular localization predicted the BocLCYb gene was in the cytoplasm and nucleus. The conserved domain of the BocLCYb protein is PLNO2463. The BocLCYb protein is most closely related to Raphanus sativus. The findings of the present study provide a molecular basis for ... More
