| Products/Services Used | Details | Operation |
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| Peptide Synthesis | Its quality and purity was ensured using NanoDrop Spectrophotometer (Thermo Fisher Scientific, USA) [OD260: OD280 nm absorption ratio (1.8–2.0)] and integrity of RNA was checked by resolving on 1.2% agarose gel containing 18% formaldehyde. An amount of ∼1 µg total RNA was reverse transcribed to first strand cDNA using random primers by Thermo Scientific Verso cDNA kit (Thermo Fisher Scientific, USA) following manufacturer's instructions. The qRT-PCR primers were designed using GenScript Real-time PCR Primer Design tool (https://www.genscript.com/ssl-bin/app/primer) (Table S1). | Get A Quote |
MYB proteins represent one of the largest transcription factor families in plants, playing important roles in diverse developmental and stress-responsive processes. Considering its significance, several genome-wide analyses have been conducted in almost all land plants except foxtail millet. Foxtail millet (Setaria italicaL.) is a model crop for investigating systems biology of millets and bioenergy grasses. Further, the crop is also known for its potential abiotic stress-tolerance. In this context, a comprehensive genome-wide survey was conducted and 209 MYB protein-encoding genes were identified in foxtail millet. All 209 S. italicaMYB (SiMYB) genes were physically mapped onto nine chromosomes of foxtail mil... More
