| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Avocado ESTs were acquired from the NCBI (http://www.ncbi.nlm.nih.gov/Entrez). Microsatellites within the expressed sequence tags were identified by the program SSRHunter (Li and Wang 2005). The repeat motifs were set as 1-6 bp, and the minimum number of repeats was ≥ 5 for di-, tri-, tetra-, penta-, and hexa nucleotide. Primers were also designed through software Primer3 (http://primer3.ut.ee/). A total of 26 EST-SSR primer pairs were developed and designated as “CATASpa”, to indicate Chinese Academy of Tropical Agricultural Sciences and Persea Americana (Tables 1 and 2). All primer pairs were produced by Genscript Biotech (Nanjing, China). | Get A Quote |
Avocado is an economically important crop and widely cultivated in the tropical and subtropical regions of China, whereas little is known about its genetic diversification. Twenty-six EST-SSR markers were developed based on avocado EST sequences. Molecular characterization was applied to assess the genetic relationships among different accessions. In total, nine gSSRs and nine EST-SSRs were selected to illuminate the genetic relationships within the 92 samples. Diversity parameters associated to the selected markers indicated the relatively low genetic diversity and a certain degree of inbreeding in the 92 avocado accessions. Clustering method and principal coordinate analysis clearly distinguished the accessio... More
