| Products/Services Used | Details | Operation |
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| Gene Synthesis | Total RNA was extracted using the RNA kit (Beijing Tiangen, China) on the basis of Manufacturer's instructions. RNA from each of the treated samples was reverse transcribed into cDNA, using the Prime Script RT reagent kit (TaKaRa, Dalian, China). Primers for selected AgbZIP genes were designed using Primer Premier 5.0 Software. Celery internal reference gene was selected as the internal control [22]. We provide the sequences of all the primers in supplementary material Table S1. Primers were synthesized by Nanjing Genscript Corporation (Nanjing China). The RT-qPCR assay was performed using the SYBR Premix Ex Taq on a CFX96 Real-time PCR system (Bio-Rad). The reaction conditions were as follows: 95 °C for 30 s followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s for annealing and extension. The experiments were performed with three independent biological replicates, and the relative expression level was calculated using the 2−ΔΔCt method | Get A Quote |
Celery (Apium graveolens L.) is one of the most important vegetables in the Apiaceae family, rich in nutrients and widely grown around the world. bZIP transcription factors family plays an important role in the transcription regulation of plant growth and development, as well as adaptation to the external environment. In this paper, 62 bZIP family transcription factors were screened and identified based on the whole genome sequence of celery. The bZIP proteins of celery and Arabidopsis thaliana were divided into 10 subfamilies according to the phylogenetic tree. Phylogenetic and evolutionary analysis showed that the number of bZIP family members gradually expanded from lower plants to higher plants during th... More
