| Products/Services Used | Details | Operation |
|---|---|---|
| Molecular Biology Reagents | Amplifications were carried out for the rDNA region of the fungal isolates using56 the ITS 5 and 4 and the TW81 and AB2857 primers. The isolated DNA was amplified in 30 µLG1 PCR mix. The reaction mixture consisted of 3 µLG1 10X PCR buffer (GenScript USA Inc), 1.5 µLG1 25 mM MgCl2, 0.6 µLG1 10 mM dNTP mix, 1.5 µLG1 10 µM of the primers (Table 1), 1 unit Taq polymerase (GenScript USA Inc) and 20 ng µLG1 genomic DNA. For the ITS region, the thermo-cycling conditions involved initial denaturation at 94EC for 3 min, followed by 35 cycles of denaturation at 94EC for 40 sec, annealing at 58EC for 40 sec and primer elongation at 72EC for 1 min followed by a final extension at 72EC for 10 min giving a product range of between 550-600 bp. | Get A Quote |
Background: Fungal endophytes are heterotrophic microorganisms that occur inside plant tissues, with some showing adverse effects against insects, nematodes and plant pathogens. Initiatives are underway at the International Center of Insect Physiology and Ecology (ICIPE) to use these endophytes as a novel strategy for control of Liriomyza leafminer. Objective: The objective of this study was therefore to search for fungal endophytes from the Liriomyza major host plants that could be used in the management of this invasive insect pest. Materials and Methods: Bioprospecting was undertaken to isolate fungal endophytes from Phaseolus vulgaris and Vicia faba seeds collected from different local and super markets in ... More
