| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | A pair of TALENs were engineered to recognize and target two 17-base pair sequences flanking the cutting site in exon 1 of the human Lhx6 gene (left TALEN: tccccggcccatgtact, right TALEN: cgggcaacgccggggcg). The constructs were made by synthetic method at Genscript. The DNA of the insertion cassette (loxP-polyA-puro-cerulean-EF1a-loxP-Citrine-2A) was synthesized at Genscript and was cloned between two 1kb homology arms of the Lhx6 gene. | Get A Quote |
The use of stem cell derived neurons for cell-based therapies is limited by a protracted maturation. We present a novel approach for accelerating the post-mitotic maturation of human stem cell derived interneurons via the activation of mTOR signaling. Lox sites were placed within PTEN, a key mTOR inhibitor, in a cortical interneuron (CIn) reporter line. Following directed differentiation and purification by FACS, the CIns were exposed to Cre-expressing lentivirus, then transplanted into mouse neocortex or plated onto cultured rat neocortex. Input synaptogenesis and dendritogenesis was greatly enhanced in the PTEN-deleted CIns. Whole-cell recording of the PTEN-deleted CIns in slices of transplanted neocortex rev... More
