| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The R5, EctP1, and EctP2 peptides (10 mg) were synthesized by Genscript (Piscataway, NJ); the genes of R5, EctP1, and EctP2 were synthesized in pUC57 (4 μg) by Genscript; pETDuet-1 was used as an expression vector from Novagen (Darmstadt, Germany); tetramethyl orthosilicate (TMOS) was employed as an silica precursor from Sigma Aldrich (St. Louis, MO); forward primer (GFP-BamHI-F: 5′-GGATCCGATGAGCAAAGGAGAA-3′) and reverse primer (GFP-PstI-R: 5′-CTGCAGTTTGTATAGTTCATCCATGCCA-3′) for polymerase chain reaction (PCR) to generate the GFP-SFP fusion proteins were synthesized by Bionics Co., Ltd. (Kangwon, Korea). | Get A Quote |
A synthetic R5 peptide showed the silica precipitation activity when added to silicic acid solution under ambient conditions. However, R5 peptide showed no silica precipitation activity below pH 7, an important pH range for biological applications. Here, we reported new silica-forming peptides (SFP), named EctP1 and EctP2. They showed better silica deposition ability than the R5 peptide. Particularly, at pH 6, EctP1 showed silica deposition activity while R5 did not. EctP1, EctP2, and R5 were genetically fused to the C-terminus of green fluorescent protein (GFP). GFP-SFP fusion proteins showed silicification ability, and GFP-EctP1 fusion protein showed the capacious silicification activity at wide pH range. The... More
